Extended Data Fig. 2: Tryptophan metabolites do not affect C. rodentium and EHEC growth and virulence in vitro.
From: Dopamine receptor D2 confers colonization resistance via microbial metabolites
C. rodentium (CR) (a–e) and EHEC O157:H7 (f–k) were cultured in the presence of I3A, IPyA, or IEt (100 μM). a, f, Growth was monitored by measuring OD600 absorbance readings over 24 h. b, g, Cultures were plated after 24 h, and CFUs were counted. (c–e, h–k) Bacteria were cultured in low glucose DMEM under anaerobic (ana), microaerophilic (micro), and aerobic (aero) conditions to activate locus of enterocyte effacement-pathogenicity island expression with I3A, IPyA, or IEt (100 μM). RNA was isolated after cultures reached late-log phase (OD600 = 0.6–0.8), and cDNA was synthesized and analyzed by qPCR for the indicated genes. Relative expression of mRNA transcripts was normalized to the RNA polymerase subunit alpha rpoA. Data are represented as the fold induction over control samples, bars = mean, error bars = standard deviation. Statistical analysis was performed using a two-tailed Student’s t-test (a–b, f–g), or one-way ANOVA, followed by post-hoc Tukey multiple comparison test (c–e, h–k), n = 3 biological replicates examined over 3 independent experiments.
