Extended Data Fig. 9: HPO-27 and MROH1 interact with RAB-7 and self-interact in a “head-to-tail” mode.
From: The HEAT repeat protein HPO-27 is a lysosome fission factor
a, Schematic illustration of the tripartite split-GFP assay. b-g’, DIC and confocal fluorescence images of the hypodermis in WT adults co-expressing GFP1-9, NUC-1::CHERRY, WT or mutant forms of GFP10::RAB-7, and HPO27::GFP11 (b-d’) or MROH1::GFP11 (e-g’). Arrowheads indicate co-localization of reconstituted GFP and lysosomes. h-s’ DIC and confocal fluorescence images of the hypodermis in WT adults co-expressing GFP1-9, HPO-27::mKate2 (h-k’, p-s’) or NUC-1::CHERRY (l-o’), and the indicated HPO-27 (h-k’), MROH1 (l-o’), HPO-27(ΔN) (p-q’), or HPO-27(ΔC) (r-s’) fusion proteins. Arrowheads indicate co-localization of reconstituted GFP with HPO-27::mKate2 or NUC-1::CHERRY. In b-s’, 15 animals were examined in each strain. Scale bars: 5 µm.
