Fig. 5: In vivo live imaging demonstrates Il10⁺ TCR^Hh T cells interacting with CD206^{+ =} macrophages in the LP.

a, CellPhoneDB analysis of receptor–ligand interactions in LP (top left) and LA (top right), restricted to cell types with more than 30 cells per region and limited to unique pairs with P < 0.01. MHCII interactions are excluded. Schematic summarizing the most relevant cell–cell interactions (bottom). b, Two-photon in vivo live imaging of TCR^HhCD2^DsRedIl10^GFP T cells transferred into Hh-colonized hosts labelled in vivo with CD206 and F4/80 fluorescent antibodies. c, Representative image of donor TCR^HhCD2^DsRedIl10^GFP T cells in the LP. Arrowheads indicate Il10⁻ TCR^Hh (red) and Il10⁺ TCR^Hh (yellow) T cells. Scale bar, 20 μm. d, Average track speed of Il10⁺ and Il10⁻ TCR^Hh T cells in the LP. e, Track displacement length of Il10⁺ and Il10⁻ TCR^Hh T cells in the LP. f, Sequential video stills showing an Il10⁺ TCR^Hh T cell moving from one CD206⁺ cell to another in the LP. Scale bars, 10 μm. g, Distance from Il10⁺ and Il10⁻ TCR^Hh to the nearest CD206⁺ cell in the LP. a,b, One sequencing run from six combined mice. d–h, Representative images and combined data from six individual mice over two independent imaging experiments. d,e,g, Mann–Whitney test.

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