Extended Data Fig. 2: TCR^Hh on a Rag1^−/− background validates specificity of TCR^Hh to Hh.

a. Frequency of Ki-67+ of TCR^Hh in the tissue (left), MLN (centre left), CP (centre right) and OLS (right) from day 1 to day 14 post cell transfer. b. Co-transfer of naive TCR^HhRag1^+/− and TCR^HhRag1^−/− into Hh-colonised hosts. c. Representative FACS plots of TCR^HhRag1^+/− and TCR^HhRag1^−/− at the indicated sites. d. Frequency of TCR^HhRag1^+/− and TCR^HhRag1^−/− of total CD4 T cells at indicated sites. e. Representative FACS plots (left) and frequency (right) of TCR^HhRag1^+/− and TCR^HhRag1^−/− Tregs of TCR^Hh CD4 T cells. f. Representative FACS plots of TCR^HhRag1^+/− and TCR^HhRag1^−/− in Hh-colonised and un-colonised hosts. a: 4 individual mice representative of 2 independent experiments. c-f: 7 individual mice representative of 2 independent experiments. d-e: two-way ANOVA using a Šidák’s multiple comparisons test with individual variances computed for each comparison.

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