Extended Data Fig. 3: FTY720 treatment supports tissue as a dominant site of TCR signalling and IL-10 production.

a. Geometric MFI (left) and frequency (right) of Nur77^GFP positive of TCR^Hh Tregs at the indicated sites. b. Frequency of IL-10+ of TCR^Hh Tregs at the indicated sites. c. Histogram overlay (left) and quantification of geometric MFI (right) of TCR^HhNur77^GFP co-cultured with Hh-pulsed BMDCs at the indicated time points. d. Transfer of TCR^HhNur77^GFP into FTY720-treated host mice. e. Representative FACS plots (left) and quantification (right) of TCRβ of total CD45 in peripheral blood. f. Geometric MFI of total TCR^HhNur77^GFP in FTY720 treated hosts at the indicated sites. g. Geometric MFI of total TCR^HhNur77^GFP in the tissue of control and FTY720 treated hosts. h. Frequency of IL-10^GFP positive among total TCR^Hh in FTY720 treated hosts. i. Frequency of IL-10^GFP positive among total TCR^Hh in the tissue of control and FTY720 treated hosts. j. Absolute TCR^Hh cell numbers in tissue of control and FTY720-treated hosts. a: 6 individual mice representative of 2 independent experiments. b: 5 individual mice representative of 2 independent experiments. c: 3 technical replicates representative of 2 independent experiments. e-j: 4 WT and 5 FTY720 treated mice representative of 2 independent experiments. a-b, f, h: one-way ANOVA using a Tukey’s multiple comparisons test. e, g, i-j: two-tailed t test.

Source Data