Extended Data Fig. 4: IL-10 is critical for maintaining homeostasis but LNs are not required for the pathogenic response to Hh in LTa^−/− mice.

a. Representative images of the caecal patch (left) and OLS (right) in Hh-colonised WT and LTa^−/− hosts. b. Representative FACS plots of TCR^Hh in tissue at 10 days post naïve TCR^Hh cell transfer. c. Absolute TCR^Hh cell count in tissue of Hh-colonised WT and LTa^−/− hosts. d. Absolute host CD4 + T cell count in tissue in Hh-colonised WT and LTa^−/− hosts. e. Representative FACS plots (left) and frequency of Tregs among TCR^Hh (right) in tissue of WT and LTa^−/− hosts at 10 days post naïve TCR^Hh transfer. f. Representative FACS plots (left) and frequency of IL-10+ among TCR^Hh (right) in tissue of WT and LTa^−/− hosts at 10 days post naïve TCR^Hh transfer. g. Absolute cell count of IL-10+ among TCR^Hh in tissue of WT and LTa^−/− hosts at 10 days post naïve TCR^Hh transfer. h. Frequency of IL-10+ of TCR^Hh Tregs in tissue of Hh-colonised WT and LTa^−/− hosts at 10 days post naïve TCR^Hh transfer. i. Colon histopathology score of Hh-colonised WT and LTa^−/− mice with and without anti-IL-10R treatment. j. Two photon in vivo live imaging of transferred naive TCR^HhCD2^dsRedNur77^GFP or TCR^HhCD2^dsRedIL-10^GFP into Hh-colonised Foxp3^GFP hosts. The caecum LA and LP were imaged. a: Representative images from 3 individual mice across 2 independent experiments. b-h: 4 WT and 4 LTa^−/− individual mice representative of 2 independent experiments. i: 5 WT and 5 LTa^−/− individual mice representative of 2 independent experiments. c-h: two-tailed t test. i: one-way ANOVA using a Tukey’s multiple comparisons test.

Source Data