Extended Data Fig. 7: Confidence for dendrograms with sorted nuclei from cortical areas.

(a) Bootstrapping results of ID01. (b) Bootstrapping results of ID05. The percentage of 10,000 replicates showing relationships between sqrt-t AFs for TBR1⁺ and DLX1⁺ nuclei in the same geographic region were more similar than TBR1⁺ nuclei from two different geographic regions. COUPTFII⁺ nuclei clustered among themselves, outside of the DLX1 and TBR1 clusters. Approximated unbiased p-value > 95% (red): the hypothesis “the cluster does not exist” rejected with a significance level (<5%). (c-d) Heatmaps and hierarchical clustering results after computational deconvolution of DLX1⁺ nuclei (grey) from Fig. 3b (c) and Fig. 3c (d). (e-f) Heatmaps and hierarchical clustering results after the simulated TBR1⁺ nuclei contamination for COUPTFII⁺ nuclear pools (black) from Fig. 3b (e) and Fig. 3c (f). (g) The estimated proportion of dorsally derived cortical inhibitory neurons within deconvolved DLX1⁺ nuclei of each lobe. The least square method is used (Methods). 11, 13 cortical lobes for ID01 and ID05, respectively. Median, thick horizontal line at the center; 95% confidence intervals, the notch of the box plot; 75 and 25% quantiles of data, upper and lower bounds of the box; Whiskers, maxima and minima excluding outliers.