Extended Data Fig. 11: FRET histogram of high and low FRET states.
From: Ligand efficacy modulates conformational dynamics of the µ-opioid receptor
FRET traces in the presence of 20 µM Gi, increasing concentrations of GDP, and ligand of TRV130, PZM21, MP, DAMGO, or BU72 were analyzed using a two-state hidden Markov Model. Only traces with at least one transition were selected. Frames of high-FRET and low-FRET states were extracted separately and binned to plot histograms. FRET histograms of high FRET (bars in orange) and low FRET (bars in green) states are shown and fitted to Gaussians (solid curves in red and blue, respectively). FRET efficiencies at 0.5 and 0.6 are highlighted with dashed lines in green and blue, respectively. n, number of fluorescence traces used to calculate the corresponding histograms. Error bars represent s.e.m. from 2 repeats.
