Extended Data Fig. 7: Analysis of gene expression in lamprey sympathetic neurons.
From: Neural crest origin of sympathetic neurons at the dawn of vertebrates
(a) Representative image of a wild-caught lamprey ammocete used for selection of trunk sympathetic neurons. Scale bar = 1 cm. (b and c) Immunohistochemical detection of TH (teal) in transverse sections through ammocete trunks. DAPI is shown in white. Yellow box indicates the site of the sympathetic chain neurons. SC=spinal cord, NC=notochord. Scale bars = 250 μm (a) and 10 μm (b). n = 4 ammocetes across three independent replicates. (d and e) GO analyses of Metabolites and Cell Compartment for TH+ neurons. Analyses are performed using the top 500 significantly upregulated transcripts with an enrichment cutoff of 1.5 log2(Fold Change). p-values are determined using Fisher’s exact test. (f) Immunohistochemical detection of DBH (teal) at T28. Punctate expression is consistent with vesicular localization of DBH protein. Scale bar = 20 μm. n = 6 embryos across three independent replicates.
