Extended Data Fig. 6: Cryo-EM based structural characterization of the Umb1 particle.

a, FSC curve with a cutoff at 0.143 for the overall Umb1 map with the 3DFSC analysis of the full particle. b, Map to model FSC curve for the final Umb1 model (in which the lectin domains of UmbA for spokes 2, 4 and 7 were not modeled due to weak density) refined against the Umb1 overall map. c, Particle distribution and local resolution of the overall Umb1 map. d, Local resolution of the unsharpened (top, threshold of 0.43) and sharpened (bottom, threshold of 1.16) Umb1 cryo-EM map. Numbers correspond to the interacting ALF repeat for each spoke. e, The final model within the unsharpened map of the Umb1 particle at a threshold of 0.43. The depiction represents the model derived from our cryo-EM data, with the exception that the lectin domains present in spokes 2, 4, and 7 are shown but were not included in the deposited model due to their weak density. f. Close up views of the density corresponding to the UmbC1 ring (threshold of 1.61, sharpened map), UmbC1 stalk (threshold of 1.16, sharpened map), and a single spoke of UmbB1 and UmbA1 (spoke 3, threshold of 1.16, local refined map).