Fig. 4: Umb particles selectively inhibit vegetative hyphal growth of Streptomyces spp.
From: Streptomyces umbrella toxin particles block hyphal growth of competing species
a, Staphylococcus aureus transformation efficiency of plasmids expressing UmbC toxin domains (1–3) relative to a vector control. Data represent the mean ± s.d. (n = 6 (experimental) or 12 (control)). b, Subset of Umb toxin susceptibility screening results. Z scores calculated from relative growth in the presence or absence of Umb toxins from two biological replicates of the screen; scores >2 indicate significant Umb-dependent inhibition. Additional strains screened shown in Extended Data Fig. 9b, and raw data provided in Supplementary Table 5. c, Growth of the indicated target and non-target strains treated with Umb or Δumb supernatant (10% (v/v), measured as relative luminescence units (r.l.u.)). Colony-forming units quantified at 16 h available in Extended Data Fig. 9c. d, Growth yields of S. griseus after 16 h of treatment with S. coelicolor Umb supernatant from the indicated strains. e, Outcome of growth competition assays between the indicated strains of S. coelicolor (Sc) and S. griseus (Sg). Data in d and e represent the mean ± s.d. (n = 3). f, Single-cell-based microscopy analysis of S. griseus growth as determined by cell area during exposure to the indicated treatments in a microfluidic flow cell. Phase I Umb supernatant-treated (sup) cells fell into two classes: those that resumed growth in toxin-free medium (growers) and those that did not (non-growers). Data for individual cells provided in Extended Data Fig. 9d and Supplementary Video 1. Shading indicates interquartile ranges. Red, n = 101; blue, n = 77; purple, n = 68; green, n = 78. g, Micrographs of representative cells from the indicated treatment groups in f outlined with Omnipose-generated segmentation masks. At 13 h, cell masks for two treatment groups are presented. Data shown in f and g are from one biological replicate representative of three. Scale bar, 2 μM. Asterisks indicate treatments significantly different from controls. P < 0.0001 (a,d) or P = 0.0003 (e), analysis of variance with Dunnett’s multiple comparisons test, two-sided.
