Extended Data Fig. 7: Spatial expression and function of Emx2 in mice.
From: Emx2 underlies the development and evolution of marsupial gliding membranes
a, Transversal sections of E11.5 laboratory mouse showing expression of Emx2 in the Wolffian ridge. b, Immunohistochemistry showing the distribution of EMX2 in the skin of PdfgraCreERT2/+/RosaEmx2-GFP/+ double transgenic animals and control littermates. c, d, Relative to control littermates, double transgenic mice over-expressing Emx2 in dermal fibroblasts show a significant increase in epidermal thickness, as seen by H&E stains (c) and cellular proliferation, as established by EdU incorporation (d). Statistical significance in panel d (N = 4; P = 0.0009) was assessed using a general mixed effects model one-way ANOVA test. Dotted line in c,d denotes the dermis-epidermis boundary. Mean + SE are plotted in d. Scale bars: 500 µm (zoomed out) and 100 µm (zoomed in) in (a); 100 µm in b-d.
