Extended Data Fig. 8: Cryo-EM workflow and analysis of the inward-facing state of MmFLVCR2.
From: Structural and molecular basis of choline uptake into the brain by FLVCR2
a, Flow chart outlining cryo-EM image acquisition and processing performed to obtain a structure of detergent-purified MmFLVCR2 in complex with the Fab FLV9. A representative micrograph and 2D class averages are shown. All processing was performed using CryoSPARC v.4.1.253 (see Methods for details). b, Euler angle distribution plot of the final three-dimensional reconstruction of the MmFLVCR2-Fab FLV9 complex. c, Fourier shell correlation (FSC) curves for the MmFLVCR2-Fab FLV9 complex. d, Local resolution map of the MmFLVCR2-Fab FLV9 complex, with an orthogonal view indicating the location of the clipping plane. Density is coloured by resolution from 2.25 (blue) to 3.25 Å (red).
