Extended Data Fig. 1: Heterogeneity primers increase nucleotide diversity during sequencing of SCI-LITE libraries.
From: Single-cell analysis reveals context-dependent, cell-level selection of mtDNA
a, Schematic of heterogeneity primer design for amplifying SCI-LITE libraries. b, Representative zoomed-in cluster images from a single cycle of Illumina sequencing, for a run with PhiX spike-in (left) and for a run with a library prepared with heterogeneity spacers (right) generated with the Illumina Sequencing Analysis Viewer. The subplots are labeled with the called base and the percentage of clusters called for each base c, Base diversity at each bp of read 1 based on sequencing data from libraries prepared with or without heterogeneity primers.
