Extended Data Fig. 2: Expression of DdCBEs correlates to heteroplasmy levels.
From: Single-cell analysis reveals context-dependent, cell-level selection of mtDNA
a, Western blots of ONC DdCBEs showing expression of FLAG-tagged right-TALE DdCBE and HA-tagged left-TALE DdCBE. Actin was used as a loading control. Data for n = 3 biological replicates are shown. b, Population doubling rate in 293 T cells transfected with DdCBEs, n = 3 biological replicates. Error bars reflect the mean ± s.d. c-d, 293 T cells were transfected with LHON DdCBE and sorted based on the fluorescence intensity of eGFP and mCherry reporters into low, medium and high groups. eGFP and mCherry intensity was used as a proxy for the expression of the editors c, Sorting strategy. Representative gating plots are shown. Graphs were generated using FCS Express software. d, Single-cell heteroplasmy measured by SCI-LITE, n = 3 biological replicates of edited cells are shown, except day 5 low where n = 2 biological replicates. Lines represent the mean for single biological replicates. Dots represent single cells.
