Extended Data Fig. 9: Single-cell RNA-seq and hybISS of testis in dysbiotic males.
From: Paternal microbiome perturbations impact offspring fitness
(a & b) Heatmap showing marker gene expression for cell identity clusters identified by unbiased analysis in mouse testis from single-cell (sc)RNA-seq analysis of ~3,000 cells, pooled from n = 4 independent males for each condition. (c) Violin plots showing the expression profiles of selected marker genes within specific cell-type clusters in mouse testis. (d) UMAP projection of single-cell RNA-seq data indicating cell-type specific proportions in testes from control or nABX-treated males. Post-meiotic cell types (highlighted) are preferentially depleted in nABX males, whereas somatic sertoli cells are proportionally enriched as a consequence. Data in each condition from n = 4 independent males pooled. (e) Violin plots showing representative examples of single-cell expression profiles of DEGs in elongating spermatids. (f) Differentially expressed genes (DEG) within each testicular cell type in nABX-exposed males. (g). KEGG pathway analysis of differentially expressed genes from elongating spermatids. q-value corrected for multiple testing. (h) In situ sequencing in single cells confirming reduction of leptin expression in the seminiferous tubules of nABX-exposed males (CON = 5; nABX=5, testes). Middle panel: bar represents the median and whiskers indicate the 5-95th percentile. Right panel: bar indicate mean with 95% C.I.
