Extended Data Fig. 6: FingR(PSD95):GFP signal intensity increases during the day and decreases at night in some, but not all tectal subtypes.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
a, Average and 68% CI of normalized synapse intensity on LD, LL, and FR conditions across one day and night for a subset of tectal neurons from Fig. 2 imaged under identical microscopy settings to enable intensity measurements. Note that the loss of the circadian clock alters the relative abundance of Type 1 and Type 2 neurons. b, Percentage change (mean and 68% CI) in normalized synapse intensity calculated as in Fig. 1. Compared to Type 2 neurons, Type 3 (p = 0.026; g = 1.777) and Type 4 (p = 0.026; g = 1.651) neurons have increased synapse intensities during the day (mixed ANOVA, interaction (subtype*time) p = 0.03, post-hoc Benjamini-Hochberg, one tailed). c, Both Type 3 (p = 0.026; g = 1.691) and Type 4 (p = 0.026; g = 1.408) neurons have significantly increased synapse intensities (with 68%CI) during the day relative to clock-break (LL) conditions (mixed ANOVA, interaction (condition*time) p = 0.006, post-hoc Benjamini-Hochberg, one tailed). Data are collected from 8 independent LD, 4 LL, and 4 FR experiments.
