Extended Data Fig. 11: FoxP2.A+ neurons express adenosine and adrenergic receptors transcripts.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
Examples of adrenergic and adenosine receptor transcripts that colocalize with labelled FoxP2.A+ neurons (middle and right panel) as detected by in situ Hybridization Chain Reaction (HCR, see Methods). a, A single labelled tectal neuron (green) colocalizes with a cocktail of HCR probes that detect adora1a-b (yellow, encoding for adenosine receptors A1a and A1b) and adora2aa, -ab, -b (magenta, encoding for adenosine receptors A2aa, A2ab, and A2b) transcripts. b, Single FoxP2.A+ neuron (green) also colocalize with an HCR probe cocktail that detects adra1 aa,-ab, -ba, -bb, -d (yellow, encoding zebrafish α1 adrenergic receptor orthologs) and adra2a, -c, -da (magenta, encoding zebrafish α2 adrenergic receptor orthologs) transcripts. Scale bar: 10 μm (a, b). Representative data from 5 larvae. Images of co-localized transcripts chosen from n = 11 neurons (a) and n = 10 neurons (b).
