Extended Data Fig. 2: The synapse number of single tectal neurons is developmentally stable at 6–9 dpf.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
a, The full map of synapse tracking from the neuron in Fig. 1c. Each column depicts a synapse, and the colour indicates the normalized GFP intensity of each synapse. In this example, 56 synapses disappeared and 20 synapses appeared during the imaging, resulting in a net change of −36 synapses. Grey bars depict night (ZT14-24). b, Example of a single FoxP2.A:FingR(PSD95)+ neuron imaged through development from 4–10 dpf. Nuclei and synapses are FingR(PSD95)-GFP+ (green), and cellular morphology is labelled by mKate2f (magenta). White arrowheads indicate examples of puncta that persisted through time. Blue arrowheads indicate examples of synapses gained/lost through time. c, Synapse counts across all neurons (average and 68% CI) (left) and for single neurons through 4–10 dpf (right). d, Average percentage change in synapse number and 68% CI calculated from the previous time point (left) and for each neuron (right). The percentage change in synapse number across time is close to zero between 6–9 dpf. n = 5 cells, 5 larvae. Scale bar: 15 μm (b).
