Extended Data Fig. 3: Example of a single FoxP2.A:FingR(PSD95)+ neuron at ZT14 and ZT18.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
a, A single FoxP2.A:FingR(PSD95)+ tectal neuron imaged at ZT14 and ZT18. Nuclei and synapses are FingR(PSD95)-GFP+ (green), and cellular morphology is labelled by mKate2f (magenta). b, Higher magnification of the primary dendrite segment (white box in a). Right panels show semi-automatic skeletonization (lines) of neurites and detection of FingR(PSD95)-GFP puncta (grey spheres, Methods). c, Higher magnification of a section of the distal arbour (white box in a). FingR(PSD95)-GFP+ puncta that appeared (blue circles and arrowheads) and disappeared (yellow circles and arrowheads) between ZT14 and ZT18 can be observed. d, Schematic showing imaging times (black arrows) at ZT14 and ZT18 on the night of 7 dpf. Scale bars: 10 ÎĽm (a) and 2.5 ÎĽm (b,c).
