Extended Data Fig. 8: (Pro)-phage targeting direct-geneFISH signal intensities.
From: Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies
Shown are the mean relative intensities of the (pro)-phage targeting direct-geneFISH signals of Roseovarius in triplicate co-culture samples withdrawn after 96 h and 120 h and the mono-culture Roseovarius samples withdrawn after 96 h as boxplots. Individual, small dots represent the (pro)-phage targeting direct-geneFISH signal intensities of the individually measured regions of interest (ROI; approximate one cell). At least 465 ROIs were defined and validated for each sample. The red dashed line defines the threshold for the definition of induced cells. The percentage below the samples indicates the proportion of induced cells in the total cells analyzed. In each treatment n = 3 biological independent samples were run. The lower and upper hinges of the box correspond to the first and third quartiles (the 25th and 75th percentiles). The central line in the box is the median. The upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles). The lower whisker extends from the hinge to the smallest value at most 1.5 * IQR of the hinge. Data beyond the end of the whiskers are called “outlying” points and are plotted individually. Prophage induction events, indicated by a significant increase of the phage signal intensity in regions of interest (ROI), were observed in 2 independent experiments.
