Extended Data Fig. 1: Growth characteristics of Colwellia and Roseovarius when supplemented with and without B12 and respective building blocks and methionine and Chaetoceros muelleri in tri-culture with Colwellia and Roseovarius.
From: Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies
a, Maximum growth yield as means of triplicates ± SD as total cell numbers per ml assessed by flow cytometry of Roseovarius; b, of Colwellia when supplemented with B12 (at 1, 10 or 100 pM) or DMB / Cbi (at 1, 10 or 100 pM); Maximum growth yield as means of triplicates ± SD as determined by optical density of c, Roseovarius and d, Colwellia. Each isolate was supplemented with B12 (100 pM), no addition (plain bar) and methionine (1 µM); e, Chlorophyll a fluorescence and total cell numbers enumerated by hemocytometer of axenic Chaetoceros muelleri over time when cultivated without any addition, f, supplemented with B12 at 10 pM and g, 100 pM final concentration and h, when grown in tri-culture with Colwellia or Roseovarius. Experiments a-d were conducted in n = 3 and experiments e-h in n = 4 biological independent samples each in 1 independent experiment.
