Extended Data Fig. 4: Growth characteristics of Thalassiosira pseudonana and Colwellia and Roseovarius cultivated in consortium.
From: Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies
a, Growth of Colwellia and Roseovarius as monitored by flow cytometric cell counts and calculated by CARD-FISH analyses. Further, numbers of virus-like particles measured by flow cytometry are presented. b, cell numbers of T. pseudonana, assessed microscopically using a hemocytometer, and bacterial cells (black circle) assessed by flow cytometry. c, Total bacterial cell abundance and the ratio of virus-like particle counts to the number of Colwellia. d, Total bacterial cell abundance and the ratio of virus-like particle counts to the number of Roseovarius. e, Relative proportions of Colwellia and Roseovarius cells (% of total abundance) during the culturing time. f. Descriptive illustration of the growth progression of the participating partners of the consortium, T. pseudonana, Roseovarius and Colwellia as well as the phage induction over a period of ten weeks. g, Maximum relative fluorescence of T. pseudonana when cultured with Colwellia and Roseovarius, with addition of 100 pM B12, without any addition, in co-culture with Roseovarius and in co-culture with Colwellia. a-f, Means of triplicates g, and ± SD. Measured values of the respective triplicates can be viewed in the corresponding source data file. Each treatment was carried out in n = 3 biological independent samples and growth characteristics were confirmed in independently conducted tests.
