Extended Data Fig. 5: Lower ligand building block concentration in the exudate of Colwellia at B12 (20 pM) depleted growth conditions and in North Sea seawater samples.
From: Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies
a, means of triplicates + SD of growth of Colwellia supplemented with 1 nM B12, 20 pM B12 and no addition, monitored by flow cytometric cell counts over time. Extracellular concentrations of α-ribazole and 5,6-dimethylbenzimidazole (DMB) were measured for the growth-limited culture when supplemented with 20 pM B12. b, concentrations of B12, cobinamide, DMB and α -ribazole in North Sea seawater samples as means of triplicates + SD. c, Map of the German Bight of the North Sea. Points mark the sampling stations of the analyzed seawater samples. Experiment a was conducted in n = 3 biological independent samples. Experiment b was conducted on n = 3 independent sampling locations and each sample was detected in n = 3 technical replicates. Experiments a-b were conducted in 1 independent experiment. The map was created using Ocean Data View.
