Extended Data Fig. 9: Structural details of the CbCas9–PcrIIC1 complex with long dsDNA.

a, Cryo-EM maps (left) and atomic models (right) of TS and sgRNA in the non-targeting complex. The Watson–Crick pairs are labelled in black and the wobble pairs are labelled in purple. b, Comparison of the DNA cryo-EM map in the CbCas9–PcrIIC1 non-targeting complex with a mimic B-form DNA model (yellow and green). The DNA region with a B-form helix was fitted using a mimic DNA backbone model (red and blue). c, Comparison of PAM interaction of preferred ACAAA PAM (left) and non-preferred CCAGA PAM (right). The hydrogen bonds are represented with blue dotted lines. d, Comparison of electrostatic interactions with the DNA by CbCas9 monomer and the CbCas9–PcrIIC1 non-targeting complex. e, Comparison of the DNA cryo-EM map in the CbCas9–PcrIIC1 dual-targeting complex with a mimic B-form DNA model (yellow and green). The DNA region with a B-form helix was fitted using a mimic DNA backbone model (red and blue).