Extended Data Fig. 5: Trajectory analysis of fetal, adult and pathological human brain vascular ECs via RNA velocity along the AV-zonation/specification axis.
From: Single-cell atlas of the human brain vasculature across development, adulthood and disease
a,f,k,p,u,z,a‘,f‘,k‘, UMAP plots of human brain ECs isolated from the indicated entities (fetal brains (21,512 ECs from 5 individuals) (a), adult/control brains (76,125 ECs from 9 individuals) (f), pathological brains (145,884 ECs from 29 individuals) (k), brain arteriovenous malformations (20,305 ECs from 5 individuals) (p), all brain tumours (125,579 ECs from 24 individuals) (u), lower-grade glioma (17,373 ECs from 6 individuals) (z) glioblastoma (49,999 ECs from 8 individuals) (a‘), brain metastasis (23,962 ECs from 5 individuals) (f‘), and meningioma (34,245 ECs from 5 individuals) (k‘)) coloured by AV specification, onto the UMAPs are superimposed RNA velocity streamlines extended by velocity-inferred directionality. b,g,l,q,v,zi,b‘,g‘,l‘, UMAP plots of the indicated entities, coloured by RNA velocity pseudotime. c,h,m,r,w,zii,c‘,h‘,m‘, UMAP plots of the indicated entities, coloured by RNA velocity length. d,i,n,s,x,ziii,d‘,i‘,n‘, UMAP plots of the indicated entities, coloured by RNA velocity confidence. e,j,o,t,y,ziv,e‘,j‘,o‘, UMAP plots of the indicated entities, coloured by AV specification, onto the UMAPs are superimposed RNA velocity PAGA vectors extended by velocity-inferred directionality.
