Extended Data Fig. 4: Alteration of AV-specification in pathological brain vascular ECs.
From: Single-cell atlas of the human brain vasculature across development, adulthood and disease
UMAP plots of human brain ECs isolated from the brain arteriovenous malformations (20,305 ECs from 5 individuals) (a,b), all brain tumours (125,579 ECs from 24 individuals) (e,f), lower-grade glioma (17,373 ECs from 6 individuals) (i,j) glioblastoma (49,999 ECs from 8 individuals) (m,n), brain metastasis (23,962 ECs from 5 individuals) (q,r), and meningioma (34,245 ECs from 5 individuals) (u,v) coloured by AV specification (a,e,i,m,q,u) and by pseudotime (b,f,j,n,r,v), the red line drawn manually indicates the major trajectory flow. c,g,k,o,s,w, Pseudotime order of ECs colour-coded according to AV specification from all theindicated entities. d,h,l,p,t,x, Heatmap of adult/control brain ECs AV specification signature gene expression in human brain ECs isolated from the indicated entities. y-ziv, Sankey plot showing the predicted annotation of the ECs of the indicated entities as mapped to adult/control brain (TL) ECs. Unassigned cells are indicated in grey. zv, Sankey plot of pathological brain ECs, showing the predicted annotation (right nodes) – as mapped to adult/control brain (TL) ECs – of the AV clusters assigned based on top cluster marker analysis (left nodes). Unassigned cells are indicated in grey. zvii, Sankey plot of fetal brain ECs, showing the predicted annotation (right nodes) – as mapped to adult/control brain (TL) ECs – of the AV clusters assigned based on top cluster marker analysis (left nodes). Unassigned cells are indicated in grey. zvi,zviii, Barplots showing the composition of unassigned cells – based on mapping to adult/control brain (TL) ECs – in pathological brain ECs (zv) and fetal brain ECs (zvii).
