Fig. 3: Retention of protein function in hydrogels.
From: Mechanical release of homogenous proteins from supramolecular gels
a, General preparation process for gel. b, Probe for aggregation inhibition using insulin with thioflavin T assay. c, Quantification of thioflavin T assay (n = 5); error bars, mean ± s.d. d, Cell-based assay for CD220 recognition by insulin following recovery from gel with shaking at 600 rpm for 24 h, compared to fresh (n = 2); error bars, mean ± s.e.m. e, The β-galactosidase assay for post-recovery function. f, Activity recovery after 7 days at 50 °C for independent repeats (n = 3); error bars, mean ± s.d. g, Comparison of 1 and 4 weeks storage and recovery of β-galactosidase (solution for 1 and 4 weeks, n = 3; gel for 1 week, n = 5; gel for 4 weeks, n = 4); error bars, mean ± s.d.
