Extended Data Fig. 2: Cryo-EM reconstruction of NET^Apo.

a, Cryo-EM data processing workflow for NET^Apo in the inward-open conformation. A representative micrograph was shown with the white bar equals 100 nm. b, Local resolution map of NET^Apo, with colors representing local resolution estimates ranging from 2.0 to 3.0 Å. c, Angular sampling of the final reconstruction, illustrating the orientation distribution of particles in the final reconstruction of NET^NA. d, Map-map and map-model Fourier shell correlations (FSC) curves. e, Electron density visualization of transmembrane helices in the NET^Apo structure.