Fig. 4: Analysis of E. faecalis phage-derived endolysin in humanized gnotobiotic GVHD mice.
From: An enterococcal phage-derived enzyme suppresses graft-versus-host disease
a, Microbial composition of the gut, on the basis of the relative abundance of operational taxonomic units at the genus level, for the donor faeces and the faecal samples from humanized mice before and after transplantation. Patient031: n = 10 for endolysin-treated mice, n = 9 for vehicle-treated mice. Patient043: n = 16 for endolysin-treated mice, n = 13 for vehicle-treated mice. Patient032: n = 8 for endolysin-treated mice, n = 8 for vehicle-treated mice. The mean of the relative abundance in each group is shown. b, Bacterial alpha diversities of faecal microbial communities using Pielou’s evenness index. Significance was determined using the Kruskal–Wallis test (two-sided). The line inside the box represents the median. The whiskers represent the range of points up to 1.5 times the interquartile range. There were some deaths in the day-15 Patient043 group and this group was therefore excluded. c, Principal coordinate analysis of the weighted UniFrac distance matrices for the faecal microbial communities. Significance was determined using a pairwise PERMANOVA. NS, not significant. Red, endolysin-treated mice; blue, vehicle-treated mice. There were some deaths in the day-15 Patient043 group and this group was therefore excluded. d, Kaplan–Meier survival plots of endolysin-treated mice and vehicle-treated mice. Significance was determined using the log-rank test (two-sided). Patient031: n = 10 for endolysin-treated mice, n = 9 for vehicle-treated mice. Patient043: n = 16 for endolysin-treated mice, n = 13 for vehicle-treated mice. Patient032: n = 8 for endolysin-treated mice, n = 8 for vehicle-treated mice.
