Extended Data Fig. 10: Subtomogram averaging of Aβ fibrils from MX04-labelled β-amyloid plaque cryo-sections.
From: CryoET of β-amyloid and tau within postmortem Alzheimer’s disease brain
Pairs of panels showing, top and side view of fibril. Left, slice (9.52 Å thick) through averaged subvolume. Right, surface rendering of subtomogram average (stalkInit, see Methods). Scale bar, 5 nm. a, Subtomogram averaging of 100 fibrils (of all widths) from one tomogram. b, Subtomogram averaging of 20 protofilament-like rods (3–5 nm diameter) from one tomogram. c, Subtomogram averaging of 42 fibrils (4–9 nm diameter) from one tomogram. d, Subtomogram averaging of 42 thick fibrils (6–12 nm diameter) from a different tomogram.
