Extended Data Fig. 6: Validation and cryo-EM data processing of reconstituted Pil1 filaments with lipid mixtures of known composition.
From: Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain
a, FRAP of TF-PI(4,5)P2 lipids in cholesterol- and ergosterol-containing lipid mixtures in the presence or absence of Pil1. b,c, FRAP of TF-PI(4,5)P2 (b) and TF-cholesterol (c) lipids in DO- or PO- lipid mixtures in the presence or absence of Pil1. In a–c, solid lines indicate a mean of n number of measured nanotubes with standard deviation shown. d, Helical reconstruction data processing strategy for reconstituted Pil1 tubules. e–h, All helical reconstructions from −PI(4,5)P2/+sterol (e), +PI(4,5)P2/−sterol (f), +PI(4,5)P2/+sterol (g) and +PI(4,5)P2/+bromosterol (h) reconstituted Pil1 tubules.
