Extended Data Fig. 10: 3D variability analysis.
From: Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain
a, 3DVA data processing strategy. b, Alignment of most compact protein lattice class (dark red) and most stretched protein lattice class (yellow) comparing measured distances between 3 different regions on neighbouring dimers within the lattice. Differences in distance between dimer regions range from 0.5–2.2 Å. c, Number of particles from each helical reconstruction type found in each 3DVA stretch class (Stretch-1=most compact protein lattice, Stretch-10=most stretched protein lattice). d, Percent of particles within each 3DVA stretch class (Stretch-1=most compact protein lattice, Stretch-10=most stretched protein lattice) deriving from each helical reconstruction type. e, One-pixel slices through unsharpened maps illustrating the lipid-binding pocket and the sterol voids of the most compact protein lattice and most stretched protein lattice 3DVA classes with the lipid-binding-pocket region indicated (most compact protein lattice: red arrows, most stretched protein lattice: yellow arrows). f, 3D surface intensity plot of sterol void slice from 5 intermediate classes along the 3DVA component exhibiting protein lattice stretch, ranging from Stretch-1 (compact protein lattice) through Stretch-9 (stretched protein lattice). g, Radial angle profile plots of bilayer within most compact protein lattice and most stretched protein lattice classes.
