Extended Data Fig. 2: Map-to-model fit.
From: Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain
a, Pil1 native-source eisosome map-to-model fit and lipid-binding pocket bound to unassigned lipid density (green). b, Lsp1 model. c, Fit comparison of divergent residues in Pil1 and Lsp1. d, −PI(4,5)P2/+sterol reconstituted map-to-model fit of unresolved AH and unoccupied lipid-binding pocket. e, +PI(4,5)P2/−sterol reconstituted map-to-model fit of AH and lipid-binding pocket bound to PI(4,5)P2 headgroup (dark green). f, +PI(4,5)P2/+sterol reconstituted map-to-model fit of AH and lipid-binding pocket bound to PI(4,5)P2 (green) and phosphatidylserine (PS; dodger blue) lipids. g, Most compact protein lattice class of 3D variability analysis (3DVA) map-to-model fit of lipid-binding pocket bound to PI(4,5)P2 (green) and putative PS (dodger blue) headgroups. h, Most stretched protein lattice class of 3DVA map-to-model fit of unoccupied lipid-binding pocket.
