Fig. 5: Effect of N-acetyltaurine administration in mice.
From: PTER is a N-acetyltaurine hydrolase that regulates feeding and obesity
a,b, Change in body weight (a) and food intake (b) of 26–28-week-old male DIO C57BL/6J mice following 7 days of treatment with the indicated dose of N-acetyltaurine (NAT; i.p.). For saline versus N-acetyltaurine (15 mg per kg per day), P = 5.95 × 10–4; for saline versus N-acetyltaurine (50 mg per kg per day), P = 6.3 × 10–4. N = 5 per group for vehicle, 1 and 5 mg per kg per day; N = 6 per group for 15 and 50 mg per kg per day. c,d, Change in body weight (c) and food intake (d) of 19–21-week-old male DIO C57BL/6J mice following treatment with the indicated metabolite at a dose of 15 mg per kg per day (i.p.). N = 5 per group. e–g, Western blots with anti-PTER (top) and anti-tubulin (bottom) antibodies (e), N-acetyltaurine hydrolysis activity (f) and tissue N-acetyltaurine levels (g) from cortex (Cort.), hypothalamus (Hyp.) and brainstem (BS) of WT mice and Pter KO mice. For WT versus Pter KO brainstem, P = 6.65 × 10–4. N = 6 per group for f and g. h, Change in 24-h food intake of 6-month-old male DIO mice treated with a single dose of GDF15 (0.1 mg kg–1, i.p.) in the presence of anti-GFRAL antibody (10 mg kg–1, i.p.) or IgG control antibody (10 mg kg–1, i.p.). N = 5 per group. i,j, Change in body weight (i) and cumulative food intake (j) of 16-week-old male DIO mice following saline or N-acetyltaurine (15 mg per kg per day, i.p.) treatment and with IgG or anti-GFRAL antibody co-treatment (10 mg kg–1, i.p., once every 3 days). N = 10 per group. Data are shown as the mean ± s.e.m. For e, the loading control was performed on the same blot. In a–d and f–h, P values were calculated from two-tailed unpaired t-tests and were not corrected for multiple comparisons. In i and j, P values were calculated from two-way ANOVA with post hoc Sidak’s multiple comparisons test. All experiments were performed once.
