Extended Data Fig. 6: Focused Ub(T) classifications in each of five conformational clusters of SCCH domain rotation in singly-loaded E1-Ub(T)-E2 complexes.
From: Structural basis for transthiolation intermediates in the ubiquitin pathway
Classifications were performed without image realignment using a focus mask on Ub(T), as shown in Extended Data Fig. 3. Overall views show maps lowpass filtered at 5 Å to better visualize Ub(T) density. The views of the adenylation site ((A) site) represent of EM density at the nominal resolution of the map superimposed with the consensus atomic model for the corresponding cluster. Isosurface levels contoured at 0.5 (5 Å lowpass filtered maps) and 0.7 ((A) site).
