Extended Data Fig. 8: Annotation of enzyme complexes in ‘Ca. M. washburnensis’ strain LCB3.
From: Methyl-reducing methanogenesis by a thermophilic culture of Korarchaeia
A. Comparison of group 1 [NiFe]-hydrogenase organization and structure between ‘Ca. M. washburnensis’ strain LCB3 and other MCR-encoding archaeal lineages. Models of the enzyme arrangement in the membrane with matching colors between the models indicating conserved function. Transmembrane helix (TMH) probabilities for intermembrane subunits. Groups 1j/1k have b-type cytochrome containing subunits with five helices, while the Korarchaeia group 1g [NiFe]-hydrogenase has an NrfD-like subunit (HcaC) with ten helices and lacks b-type cytochromes. B. Annotation of the ‘Ca. M. washburnensis’ strain LCB3 HdrDE complex. Model representing the arrangement of genes in the membrane. All HdrE subunits analyzed have 5 TMHs. The HdrD subunit in the LCB3 genome has cysteine residues that are conserved in HdrD subunits from other lineages. Transmembrane helices were predicted using the TMHMM 2.0 server.
