Extended Data Fig. 4: Representative fluorescence micrographs of culture LCB3.
From: Methyl-reducing methanogenesis by a thermophilic culture of Korarchaeia
A. Cells visualized with DAPI (blue). B. Cells visualized by DOPE-FISH using a ‘Ca. M. washburnensis’ specific 16S rRNA-targeted oligonucleotide probe (KRmw515, red). C. Overlay of A and B. D. Cells visualized with DAPI (blue). E. Cells visualized with DAPI (blue, same field of view as in D) combined with dual CARD-FISH using the ‘Ca. M. washburnensis’ specific 16S rRNA-targeted oligonucleotide probe (KRmw515, red) and a general archaea probe (Arch915, green). Note that because of two mismatches of the Arch915 probe to the 16S rRNA of ‘Ca. M. washburnensis’ there is no overlap of signal from probes KRmw515 and Arch915. Representative micrographs from n = 3 independent samples from culture LCB3. Scale bars 5 µm.
