Extended Data Fig. 5: Methane production in culture LCB3 under different experimental conditions.
From: Methyl-reducing methanogenesis by a thermophilic culture of Korarchaeia
A. Methane production curves for SIP incubations (shown in Fig. 1b) amended with 12C-methanol (open white circles), 13C-methanol (gray filled circles), or 13C-methanol with BES (black filled circles). Data are presented as mean values +/− standard deviation (n = 3 biological replicates). B. Methane production curves for BONCAT-FISH incubations (shown in Fig. 3c) amended with methanol and hydrogen, spiked with BES at day 15 (filled symbols, dashed line) and spiked with HPG at day 18 (filled symbols). Controls were not spiked (open symbols), representing growth under standard cultivation conditions, or were spiked with BES at day 0 (open symbols, dashed line), inhibiting methane production. Incubations were sampled for cell visualization via BONCAT-FISH after 24 h of incubation with HPG (day 19). Data are presented as mean values +/− standard deviation (n = 3 biological replicates). C. Methane produced with different substrates after 85 days of incubation. Concentrations were 10 mM for methanol (MeOH), monomethylamine (MMA), dimethylamine (DMA), trimethylamine (TMA), ethanol (EtOH), and isopropanol (Prop); 2mM for lactate (Lac); and 50% for hydrogen (H2).
