Fig. 5: EAG analysis to address whether heat activation is impaired in the trpA1 and opsin mutants.
From: Thermal infrared directs host-seeking behaviour in Aedes aegypti mosquitoes
a, IR thermograph of the thermal output of the 4 cm × 4 cm × 1 cm IR source used in the experiments. Right, the 4 cm × 4 cm × 1 cm block was held at around 34 °C. Left, the same block at ambient temperature (around 21 °C). The images were acquired using the FLIR One IR smartphone camera. b, The temperature at the site of the antennal preparation before exposure to the 34 °C block and during exposure to the 34 °C IR stimulus (grey shading). The temperature was measured by placing the tip of the probe (TSP01-USB Temperature and Humidity Data Logger; Thor Labs) at the same site as the antennal preparation used for the EAG experiments. c, Representative EAG traces from wild-type (control), trpA11 and op12,op21 female mosquitos that were presented with the IR source (grey shading). d, EAG amplitudes exhibited by wild-type (control), trpA11 and op12,op21 mosquitos in response to a 34 °C IR source. n = 12 (wild-type control and trpA11) and n = 11 (op12,op21). Each datapoint represents EAG responses from individual mosquitoes. Data are mean ± s.e.m. Statistical analysis was performed using one-way aligned-rank ANOVA with pairwise contrasts (d); **P < 0.01. Exact P values are provided as source data.
