Extended Data Fig. 3: TNF production by the patients’ primary cells.
From: Tuberculosis in otherwise healthy adults with inherited TNF deficiency
a, Gating strategy related to Fig. 2e for identification of the dendritic cell (DC) and monocyte subsets. b, TNF production by classical monocytes from a local control (Ctrl), P1 and P2 and the healthy brother of P1 without (NS) and with stimulation with LPS, BCG or L. monocytogenes (L.m). c, TNF secretion into whole blood after stimulation with BCG or IFN-γ for healthy controls (n = 22), P1 and P2. d, IL-6 secretion by total PBMCs from P1, her brother and healthy controls (n = 5) after stimulation with LPS, BCG or L.m. Error bars indicate the mean ± s.d. e, Frequency of TNFR1+ and TNFR2+ cells in the indicated subsets of PBMCs from healthy controls (n = 8), and TNF-deficient patients. Error bars indicate the mean ± s.d. f, Frequency of TNF+ cells in the indicated subsets of PBMCs with and without stimulation with BCG, for healthy controls (n = 22), P1 and P2. mDC, myeloid dendritic cells; MAIT, mucosal-associated invariant T cells; iNKT, invariant natural killer T cells.
