Extended Data Fig. 4: Deep immunophenotyping by spectral flow cytometry for TNF-deficient patients.
From: Tuberculosis in otherwise healthy adults with inherited TNF deficiency
Frequency of (a) CD4+ T cells and CD8+ T cells and their subsets, (b) double-negative (DN), double-positive (DP), T-cell receptor (TCR) αβ+ and regulatory T cells (Treg), (c) T helper (Th) cells and their subsets, (d) TCR γδ+ T cells, (e) mucosal-associated invariant T cells (MAIT), (f) innate lymphoid cells (ILC), (g) NK cells and their subsets, (h) B cells and plasma cells, (i) dendritic cell (DC) subsets, (j) and monocytes and their subsets in healthy adult controls (n = 36), healthy, age-matched controls from Colombia (n = 10), P1 and P2, as determined by spectral flow cytometry. CM, central memory; EM, effector memory; TEMRA, effector memory re-expressing CD45RA, ILCP, innate lymphoid cell precursor, iNKT, invariant natural killer T cells; mDC, myeloid dendritic cells; cDC, conventional dendritic cells; pDC, plasmacytoid dendritic cells. Significance was assessed using two-tailed Mann-Whitney U tests (a, c, e, f, g and i).
