Extended Data Fig. 4: AriB toxicity is dependent on association with AriA.
From: A virally encoded tRNA neutralizes the PARIS antiviral defence system
a, Liquid culture toxicity assays of AriB alone or AriA:B together (PARIS). Genes are overexpressed using a T7 inducible promoter. AriB alone results in a mild growth defect, while Ocr-triggered release of AriB (PARIS Ocr) is significantly more toxic. b, Growth of E. coli MG1655 carrying plasmid pFR85 (pSC101 with ariAB under the control of the native promoter), or variants with ariA deleted, ariB deleted or a stop codon in ariA yielding an interrupted protein that lacks the interaction interface with AriB. c, Structure-guided mutations that disrupt the AriA:AriB interface result in a loss of defense phenotype. d, Drop-test toxicity assay with PARIS (WT) or PARIS with AriA D401N/E404Q mutation. A modest growth defect can be observed only for the PARIS AriA D401N/E404Q overexpressed from T7 promoter. e, The addition of pBAD Ocr to the PARIS AriA mutants does not increase the toxicity, indicating that AriB is not functional when AriA interactions are disrupted. f, Close-up of the AriA:AriB interaction interface. Mutated residues are labeled with asterisks.
