Extended Data Fig. 7: AriA ATPase activity.
From: A virally encoded tRNA neutralizes the PARIS antiviral defence system
a, PARIS mediated hydrolysis of α32P ATP with PARIS alone or with 10-fold excess trigger (T7 Ocr). Reactions were run from 1 to 32 min and products were resolved via thin layer chromatography. Reactions with α32P ATP incubated with T4 polynucleotide kinase were used as a positive control. Images are representative of reactions that were performed in triplicate. b, Ocr alone was incubated with α32P ATP for 32 min indicating that the trigger alone does not hydrolyze ATP. c, Concentration of ADP formed at each time point was quantified and plotted. Data points of three technical replicates are shown.
