Extended Data Fig. 11: tRNALys cleavage product and phylogenetics of PARIS.
From: A virally encoded tRNA neutralizes the PARIS antiviral defence system
a, Alignment of the B803 and B806 Northern Blot probes to the E. coli and T5 tRNALys. b, Northern Blot with probe B806 in the presence or absence of the T5 tRNALys expressed from a pBAD. c, Mapping of the tRNALys cleavage site by reverse-transcription, adapter ligation, PCR and Sanger sequencing. The cleavage site, identified as the junction between the tRNA sequence and the ligated adapter, is marked with a vertical red line. d, Alignment of homologs of AriA representative of the different PARIS clades. Different domains of AriA are indicated. Grey scale represents % of identity between homologs. e, Phylogenetic tree of DUF4435 domain of AriB using M5 Ribonuclease (TOPRIM) as an outgroup.
