Extended Data Fig. 7: Spatial and size distribution of wild-type confetti clones in the mammary gland.
From: Mechanisms that clear mutations drive field cancerization in mammary tissue
a, Representative confocal overview image of a whole mount 5th mammary gland after 550 days of lineage tracing, illustrating the distribution of wild-type confetti clones within the ductal tree. Images depict 3D-rendering of Z-stacks, with the confetti labelled cells in their respective colour and the mammary ducts labelled with Keratin 14 (KRT14) shown in white. Scale bars represent 1 mm (left panel), 100 µm (panel 1 and 2), and 50 µm (panel 3 and 4). Representative image of n = 8 glands from 4 biological repeats (mice). b, Branch levels are defined as the number of branch points starting from the main duct close to the nipple. c, Quantification of the wild-type confetti clone size by branch level. Each dot represents a clone, cyan dots for luminal clones and blue dots for basal clones. Line indicates linear regression of the luminal and basal clone sizes with R2, slope and 95% confidence interval of the slope indicated in the graph, n = 6 glands from 3 mice. d, Number of wild-type confetti clones represented by bars for each branch level after 550 days of tracing in n = 6 glands from 3 mice.
