Extended Data Fig. 1: Quality measures and marker expression.
From: DNA methylation controls stemness of astrocytes in health and ischaemia
a, scNMT-seq quality metrics for all cells that passed quality filtering. “Unique genes” refers to the number of genes with at least one sequencing read per cell. “CpG/GpC-dinucleotides” refers to the number of methylation sites with sequencing coverage and thus known methylation status (large circle: median; error bar: interquartile range). For quality metrics of all cells reported in this study, see Supplementary Fig. 1. b, Gene expression of key marker genes and transcription factors along pseudotime. c, Correlation of promoter methylation (left) and promoter accessibility (right) with gene expression across cells. Negative correlations (blue) indicate a repressive effect of methylation/accessibility; positive correlations (orange) indicate activation. n: number of nominally significant correlations (Pearson’s two-sided correlation test) after Benjamini-Hochberg adjustment; y axis: unadjusted p-values.
