Fig. 3: BRCA1–BARD1 together with CtIP and MRN promote resection by WRN-DNA2-RPA.
From: Mechanism of BRCA1–BARD1 function in DNA end resection and DNA protection
a, Representative kinetic resection assays with WRN-DNA2-RPA, in the absence or presence of pCtIP and BRCA1–BARD1. b, Representative resection assays with BLM-DNA2-pCtIP-RPA, in the absence or presence of BRCA1–BARD1. c, DNA unwinding by the indicated proteins, at 25 nM, expressed as ΔDNA-length (Methods). Averages shown; error bars, s.e.m.; n = 7 (for WRN), n = 6 (for WRN-BRCA1–BARD1), n = 7 (for WRN-BRCA1–BARD1-pCtIP), n = 9 (for WRN-MRN-BRCA1–BARD1), n = 5 (for WRN-pCtIP-MRN). d, Representative resection assays with WRN-DNA2-pCtIP-BRCA1–BARD1-RPA, in the absence or presence of λ phosphatase (200 U). e, Representative resection assays with WRN-DNA2-RPA, in the presence of pCtIP or dephosphorylated CtIP (λCtIP) and in the absence or presence of BRCA1–BARD1. f, Representative resection assays with WRN-DNA2-pCtIP-RPA, in the absence or presence of either BRCA1–BARD1 (BRCA1–BARD1) or dephosphorylated BRCA1–BARD1 (λBRCA1–BARD1). Source data are provided in Supplementary Fig. 2. knt, kilonucleotides.
