Extended Data Fig. 8: Pharmacologically targeting neurofibrosis promotes weight loss in obesity.
From: Pathogenic hypothalamic extracellular matrix promotes metabolic disease
a) 12-week HFHS fed male C57BL/6 J mice received daily I.C.V. administration of vehicle or fluorosamine for 10 days. PNN expression in the b-d) ARC, e,f) RSG and g,h) habenula was assessed. Effects on i) body weight, j,k) adiposity, l) energy expenditure, m) ambulatory activity, and n) food intake was assessed. HFHS fed mice received I.C.V. administration of vehicle or fluorosamine (100 or 250 μg/animal) for 10 consecutive days, fasted overnight then given access to food ad libitum and o) behaviour, p) transition point from feeding to resting/satiety and q) the time to satiety was determined. Results are mean ± SEM. Significance determined c, d, h, k, l, n, q) one-way ANOVA with Tukey’s multiple comparisons, i) two-way ANOVA with multiple comparisons. n = biologically independent samples and significance is representative of at least two independent experiments. Scale bar, 100 µm. Abbreviations: III, 3rd ventricle; HFHS, high-fat high-sugar diet; I.C.V., intracerebroventricular; PNN, perineuronal net; WFA, Wisteria Floribunda Lectin.
