Fig. 3: Self-organized tissue mechanics drives ectopic embryo formation after epiblast subdivision.
From: Self-organized tissue mechanics underlie embryonic regulation
a–e, Model predictions for the response to epiblast bisection in anterior halves. a, Sketch of epiblast bisection. b–e, Predicted contractility (magenta), tension (green) and velocity (black) profiles at t0 + 4 h (b), kymograph of margin strain rate (c; 0 mm is anterior) and deformation maps at 4 h (d) and 8 h (e) after bisection. f–w, UV-laser dissected anterior epiblast halves with (f–k) or without (l–w) epiboly and treated with H1152 (r–w). The red dashed lines indicate the UV cut abrogating the epiboly process. f,l,r, A memGFP embryo at t0. g,m,s, Kymographs of the strain rates along the margin; 0 mm is anterior. h,n,t, Deformation maps at 10 h (h), 15 h (n) and 13 h (t) after epiblast bisection. i,o,u, SNAI2 expression in the same embryos fixed after live imaging (n = 8 out of 8 biologically independent embryos with epiboly; n = 5 out of 5 without epiboly (3 out of 5 show two ectopic primitive streaks, 2 out of 5 show one ectopic primitive streak); n = 4 out of 4 without epiboly and with H1152). j,k,p,q,v,w, GDF1 expression and the corresponding deformation maps at 4.5 h (j,k,p,q) and 6 h (v,w) after epiblast bisection. Scale bars, 1 mm (f, h–k, l, n–q, r and t–w).
